Single-stranded DNA is currently being produced by several different methods including asymmetric PCR. ... Open in figure viewer PowerPoint… Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. It reduces nonspecific binding of Products. Asymmetric PCR differs from regular PCR by the excessive amount … Procedure of Nested PCR The primers used for hiTAIL-PCR are shown in Figure 1.. PCR … Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR … Nested PCR is the improvement of polymerase chain reaction was design to improve specificity. Nested PCR used two sets of Primers. This is done by limiting or leaving out one of the primers. The PCR product is purified by centrifugation through a Millipore Ultrafree-DA centrifugal filter device … It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required. Technol. many types of PCR techniques such as RT-PCR, touchdown PCR, real time PCR, nested PCR, multiplex PCR, semi quantitative PCR, assembly PCR, asymmetric PCR, LATE- PCR, dial out-PCR etc., This paper is an attempt to give a brief idea about the various types of PCR techniques Keywords: PCR-Technique, Applications of PCR, Review of PCR. Figure 1. 2012; 34: 125-131 (Free full text). PCR Primers. Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. One used in the first reaction of polymerase chain reaction and 2nd used in the product of the first reaction to amplifying the purpose. Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Basic PCR techniques • Asymmetric PCR: preferentially amplifies one DNA strand in a double-stranded DNA template. Primers used for high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR). The results demonstrate that our molecular‐beacon‐based asymmetric PCR assay is an easy, reliable, high‐yield, and cost‐effective method for the simultaneous detection of three polymorphisms related to folate metabolism. Rapid-cycle real-time PCR by the LightCycler (Roche Molecular Biochemicals, Auckland, New Zealand) provides a very quick, one-step means of genotyping herpes simplex virus (HSV) from clinical samples … Songklanakarin J. Sci. 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